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Experimental Data by iGEM British Columbia 2013

4-CMH (BBa_K1129046) under the constitutive promoter (BBa_J23118) and (BBa_K1129043) under an arabinose inducible promoter (BBa_I13453)were transformed into E.coli 10G cells. An overnight culture of these cells harboring (BBa_K1129046) was inoculated into a fresh culture of 5mL minimal media containing tyrsoine. Cells and were harvested for GC-MS after 7 hours of growth.

Figure 3. Compound generation identification by GC-MS. Chromatograms and mass spectra for select peaks are shown. Structures represent predictions based on library matching or comparison to standards. Controls represent plasmids missing the gene of interest. A) Internal control using caffeic acid. B) Conversion of p-coumaric acid to caffeic acid by constitutively expressed 4-CMH. C) Possible conversion of p-coumaric acid to caffeic acid after induction by arabinose on 4CMH under the control by the arabinose promoter. The mass spectrum however is confounded by another compound.

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